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boronophenylalanine bpa uptake assay kit  (Dojindo Labs)


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    Structured Review

    Dojindo Labs boronophenylalanine bpa uptake assay kit
    Analysis of <t>BPA</t> uptake. (A) BPA uptake in plated cells exposed to 8 Gy IR was analyzed using a microplate reader. Data are presented as the mean ± SD of the ratio of the IR group (n=6) relative to the control group (n=10). (B) Representative histogram of BPA uptake in single cells analyzed by flow cytometry. (C) Quantification of BPA uptake in single cells (n=3). *P<0.01 (unpaired Student's t-test). BPA, <t>boronophenylalanine;</t> IR, ionizing radiation.
    Boronophenylalanine Bpa Uptake Assay Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bpa+uptake+assay/Amino+Acid+Uptake+Assay+Kit/pmc12489427-108-9-17
    Average 93 stars, based on 18 article reviews
    boronophenylalanine bpa uptake assay kit - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Relationship between amino acid transporter activity and radioactive iodine therapy efficacy in differentiated thyroid cancer"

    Article Title: Relationship between amino acid transporter activity and radioactive iodine therapy efficacy in differentiated thyroid cancer

    Journal: Oncology Reports

    doi: 10.3892/or.2025.8993

    Analysis of BPA uptake. (A) BPA uptake in plated cells exposed to 8 Gy IR was analyzed using a microplate reader. Data are presented as the mean ± SD of the ratio of the IR group (n=6) relative to the control group (n=10). (B) Representative histogram of BPA uptake in single cells analyzed by flow cytometry. (C) Quantification of BPA uptake in single cells (n=3). *P<0.01 (unpaired Student's t-test). BPA, boronophenylalanine; IR, ionizing radiation.
    Figure Legend Snippet: Analysis of BPA uptake. (A) BPA uptake in plated cells exposed to 8 Gy IR was analyzed using a microplate reader. Data are presented as the mean ± SD of the ratio of the IR group (n=6) relative to the control group (n=10). (B) Representative histogram of BPA uptake in single cells analyzed by flow cytometry. (C) Quantification of BPA uptake in single cells (n=3). *P<0.01 (unpaired Student's t-test). BPA, boronophenylalanine; IR, ionizing radiation.

    Techniques Used: Control, Flow Cytometry

    Related Articles

    Membrane:

    Article Title: The Legionella pneumophila type IVb secretion system effector BinA subverts amino acid transport to sensitize TORC1 signaling in macrophages
    Article Snippet: BPA - a phenylalanine derivative - is internalized via the amino acid transporter LAT-1.BPA - a phenylalanine derivative - is internalized via the amino acid transporter LAT-1.. BPA uptake assay (Dojindo Labratories, cat# UP04) was used to measure BPA transport in Lp-infected macrophages as per the suggested manufacturer’s protocol.. Briefly, 1×10 5 Myd88 KO iBMDMs were seeded in each well of a 96-well black wall microplate and were cultured at 37°C overnight in a 5% CO2 incubator in complete DMEM medium.Briefly, 1×10 5 Myd88 KO iBMDMs were seeded in each well of a 96-well black wall microplate and were cultured at 37°C overnight in a 5% CO2 incubator in complete DMEM medium.

    Infection:

    Article Title: The Legionella pneumophila type IVb secretion system effector BinA subverts amino acid transport to sensitize TORC1 signaling in macrophages
    Article Snippet: BPA - a phenylalanine derivative - is internalized via the amino acid transporter LAT-1.BPA - a phenylalanine derivative - is internalized via the amino acid transporter LAT-1.. BPA uptake assay (Dojindo Labratories, cat# UP04) was used to measure BPA transport in Lp-infected macrophages as per the suggested manufacturer’s protocol.. Briefly, 1×10 5 Myd88 KO iBMDMs were seeded in each well of a 96-well black wall microplate and were cultured at 37°C overnight in a 5% CO2 incubator in complete DMEM medium.Briefly, 1×10 5 Myd88 KO iBMDMs were seeded in each well of a 96-well black wall microplate and were cultured at 37°C overnight in a 5% CO2 incubator in complete DMEM medium.

    Control:

    Article Title: The Legionella pneumophila type IVb secretion system effector BinA subverts amino acid transport to sensitize TORC1 signaling in macrophages
    Article Snippet: BPA - a phenylalanine derivative - is internalized via the amino acid transporter LAT-1.BPA - a phenylalanine derivative - is internalized via the amino acid transporter LAT-1.. BPA uptake assay (Dojindo Labratories, cat# UP04) was used to measure BPA transport in Lp-infected macrophages as per the suggested manufacturer’s protocol.. Briefly, 1×10 5 Myd88 KO iBMDMs were seeded in each well of a 96-well black wall microplate and were cultured at 37°C overnight in a 5% CO2 incubator in complete DMEM medium.Briefly, 1×10 5 Myd88 KO iBMDMs were seeded in each well of a 96-well black wall microplate and were cultured at 37°C overnight in a 5% CO2 incubator in complete DMEM medium.

    Fluorescence:

    Article Title: The Legionella pneumophila type IVb secretion system effector BinA subverts amino acid transport to sensitize TORC1 signaling in macrophages
    Article Snippet: BPA - a phenylalanine derivative - is internalized via the amino acid transporter LAT-1.BPA - a phenylalanine derivative - is internalized via the amino acid transporter LAT-1.. BPA uptake assay (Dojindo Labratories, cat# UP04) was used to measure BPA transport in Lp-infected macrophages as per the suggested manufacturer’s protocol.. Briefly, 1×10 5 Myd88 KO iBMDMs were seeded in each well of a 96-well black wall microplate and were cultured at 37°C overnight in a 5% CO2 incubator in complete DMEM medium.Briefly, 1×10 5 Myd88 KO iBMDMs were seeded in each well of a 96-well black wall microplate and were cultured at 37°C overnight in a 5% CO2 incubator in complete DMEM medium.

    Comparison:

    Article Title: The Legionella pneumophila type IVb secretion system effector BinA subverts amino acid transport to sensitize TORC1 signaling in macrophages
    Article Snippet: BPA - a phenylalanine derivative - is internalized via the amino acid transporter LAT-1.BPA - a phenylalanine derivative - is internalized via the amino acid transporter LAT-1.. BPA uptake assay (Dojindo Labratories, cat# UP04) was used to measure BPA transport in Lp-infected macrophages as per the suggested manufacturer’s protocol.. Briefly, 1×10 5 Myd88 KO iBMDMs were seeded in each well of a 96-well black wall microplate and were cultured at 37°C overnight in a 5% CO2 incubator in complete DMEM medium.Briefly, 1×10 5 Myd88 KO iBMDMs were seeded in each well of a 96-well black wall microplate and were cultured at 37°C overnight in a 5% CO2 incubator in complete DMEM medium.



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    boronophenylalanine bpa uptake assay kit  (Dojindo Labs)
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    Dojindo Labs boronophenylalanine bpa uptake assay kit
    Analysis of <t>BPA</t> uptake. (A) BPA uptake in plated cells exposed to 8 Gy IR was analyzed using a microplate reader. Data are presented as the mean ± SD of the ratio of the IR group (n=6) relative to the control group (n=10). (B) Representative histogram of BPA uptake in single cells analyzed by flow cytometry. (C) Quantification of BPA uptake in single cells (n=3). *P<0.01 (unpaired Student's t-test). BPA, <t>boronophenylalanine;</t> IR, ionizing radiation.
    Boronophenylalanine Bpa Uptake Assay Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bpa uptake assay  (Dojindo Labs)
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    Dojindo Labs bpa uptake assay
    (A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
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    prewarmed bpa uptake solution  (Dojindo Labs)
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    (A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
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    Image Search Results


    Analysis of BPA uptake. (A) BPA uptake in plated cells exposed to 8 Gy IR was analyzed using a microplate reader. Data are presented as the mean ± SD of the ratio of the IR group (n=6) relative to the control group (n=10). (B) Representative histogram of BPA uptake in single cells analyzed by flow cytometry. (C) Quantification of BPA uptake in single cells (n=3). *P<0.01 (unpaired Student's t-test). BPA, boronophenylalanine; IR, ionizing radiation.

    Journal: Oncology Reports

    Article Title: Relationship between amino acid transporter activity and radioactive iodine therapy efficacy in differentiated thyroid cancer

    doi: 10.3892/or.2025.8993

    Figure Lengend Snippet: Analysis of BPA uptake. (A) BPA uptake in plated cells exposed to 8 Gy IR was analyzed using a microplate reader. Data are presented as the mean ± SD of the ratio of the IR group (n=6) relative to the control group (n=10). (B) Representative histogram of BPA uptake in single cells analyzed by flow cytometry. (C) Quantification of BPA uptake in single cells (n=3). *P<0.01 (unpaired Student's t-test). BPA, boronophenylalanine; IR, ionizing radiation.

    Article Snippet: Amino acid uptake into cells was analyzed using a boronophenylalanine (BPA) uptake assay kit (cat. no. 342-09893; Dojindo Laboratories, Inc.).

    Techniques: Control, Flow Cytometry

    (A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine (L-BPA). Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- BPA uptake by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.

    Journal: bioRxiv

    Article Title: The Legionella pneumophila type IVb secretion system effector BinA subverts amino acid transport to sensitize TORC1 signaling in macrophages

    doi: 10.1101/2025.02.20.639245

    Figure Lengend Snippet: (A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine (L-BPA). Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- BPA uptake by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.

    Article Snippet: BPA uptake assay (Dojindo Labratories, cat# UP04) was used to measure BPA transport in Lp-infected macrophages as per the suggested manufacturer’s protocol.

    Techniques: Membrane, Infection, Control, Fluorescence, Comparison